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spectral flow cytometry  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences spectral flow cytometry
    Spectral Flow Cytometry, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 4408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spectral+flow+cytometry/Aurora/pm41896813-173-9-12
    Average 99 stars, based on 4408 article reviews
    spectral flow cytometry - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Neutrophil-microglia interaction drives motor dysfunction in neuromyelitis optica model induced by subarachnoid AQP4-IgG
    Article Snippet: To deplete peripheral neutrophils, mice were injected twice i.p. with anti-mouse Ly6G (InVivoMAb, BioXCell, BE0075-1, Clone: 1A8, 100 mg/kg) or isotype control rat IgG2a, at 5 and 3 days before rotarod testing. .. The following antibodies identified mature neutrophils (Gr1hiCD11b+, GrhiMPO+) (65), immature myeloid cells (Gr1in/CD11b+) (65), immature neutrophils or granulocytic precursors (Gr1int/MPO+) (66, 67) in peripheral blood using spectral flow cytometry (Cytek Aurora, Cytek Biosciences): CD45-violetFluorTM 450 (Tonbo, 75- 0451-U100, Clone 30-F11, 1:1,000), CD45-PE-CF594 (BD, 562420, Clone 30-F11, 1:1,000), CD11b-PE-Cy5 (Tonbo, 55-0112-U100, Clone M1/70, 1:1,000), Ly6G-APC-Cy7 (BioLegend, 127623, Clone 1A8, 1:200), Ly6C-PerCP (BioLegend, 128028, Clone HK1.4, 1:100), Gr1-PE (BioLegend, 108408, Clone RB6-8C5, 1:200). .. After exposure to surface antibody panel, cells were washed, permeabilized by Cytofix/Cytoperm solution (BD #554722) at 4 °C for 20 min, then exposed sequentially to polyclonal goat anti-MPO IgG (R&D, AF3667, 1:1,000; 30 min at 4 °C), washed then donkey anti-goat IgG (Alexa Fluor 488-conjugated, Invitrogen, A32814TR; 30 min at 4 °C); both antibodies were diluted in 1× Perm/Wash buffer (BD #554723).

    Article Title: Erythropoiesis–inosine metabolic axis failure underlying retinal neurodegeneration in glaucoma: novel diagnoses and therapies
    Article Snippet: .. Following staining, the cells were washed with flow buffer and resuspended in 100 μl of flow buffer, and analyzed within 1 h using spectral flow cytometry (Cytek Northern Lights). .. Mix I included Pacific Blue-conjugated lineage markers (BioLegend 133310), c-KIT (BD 553355), Sca-1 (BioLegend 108134), CD16/32 (BD 570676), CD34 (BD 560230), CD135 (BD 562537) and CD127 (BD 560733).

    Article Title: Neutrophil-microglia interaction drives motor dysfunction in a neuromyelitis optica model induced by subarachnoid AQP4-IgG
    Article Snippet: To deplete peripheral neutrophils, mice were injected twice i.p. with anti-mouse Ly6G ( InVivo MAb, Bio X Cell, BE0075-1, clone: 1A8, 100 mg/kg) or isotype control rat IgG2a, at 5 and 3 days before rotarod testing. .. The following antibodies identified mature neutrophils (Gr1 hi CD11b + , Gr hi MPO + ) , immature myeloid cells (Gr1 int CD11b + ) , and immature neutrophils or granulocytic precursors (Gr1 int MPO + ) ( , ) in peripheral blood using spectral flow cytometry (Cytek Aurora, Cytek Biosciences): CD45-violetFluor 450 (Tonbo, 75-0451-U100, clone 30-F11, 1:1,000), CD45-PE-CF594 (BD Biosciences, 562420, clone 30-F11, 1:1,000), CD11b-PE-Cy5 (Tonbo, 55-0112-U100, clone M1/70, 1:1,000), Ly6G-APC-Cy7 (BioLegend, 127623, clone 1A8, 1:200), Ly6C-PerCP (BioLegend, 128028, clone HK1.4, 1:100), and Gr1-PE (BioLegend, 108408, clone RB6-8C5, 1:200). .. After exposure to surface antibody panel, cells were washed, permeabilized by Cytofix/Cytoperm solution (BD Biosciences 554722) at 4°C for 20 min, then exposed sequentially to polyclonal goat anti-MPO IgG (R&D Systems, AF3667, 1:1,000; 30 min at 4°C), then donkey anti-goat IgG (Alexa Fluor 488–conjugated, Invitrogen, A32814TR; 30 min at 4°C); both antibodies were diluted in 1× Perm/Wash buffer (BD Biosciences 554723).

    Article Title: IFNγ signaling drives resistance to FLT3 inhibition in acute myeloid leukemia
    Article Snippet: .. Spectral flow cytometry was conducted using the Cytek Aurora (Cytek Biosciences, CA). ..

    Article Title: Inflammatory and immune profiling in children and adolescents with attention-deficit/hyperactivity disorder (ADHD): a matched case-control study with longitudinal on/off psychostimulant assessment (ANIME).
    Article Snippet: .. The INRAE team will perform peripheral immune phenotyping using spectral flow cytometry (Cytek Aurora) with a predefined panel of markers targeting major immune cell populations. ..

    Article Title: Erythropoiesis-inosine metabolic axis failure underlying retinal neurodegeneration in glaucoma: novel diagnoses and therapies.
    Article Snippet: .. Following staining, the cells were washed with flow buffer and resuspended in 100 μl of flow buffer, and analyzed within 1 h using spectral flow cytometry (Cytek Northern Lights). .. Mix I included Pacific Blue-conjugated lineage markers (BioLegend 133310), c-KIT (BD 553355), Sca-1 (BioLegend 108134), CD16/ 32 (BD 570676), CD34 (BD 560230), CD135 (BD 562537) and CD127 (BD 560733).

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro.
    Article Snippet: .. Fluorescence was then acquired using spectral flow cytometry (Cytek Northern Lights, Cytek; Fremont, CA) and analyzed using FlowJo (version 10.10). ..

    Article Title: Interleukin-1 receptor-1 signaling mediates neuroinflammation, neuronal injury, and cognitive decline after diffuse traumatic brain injury.
    Article Snippet: Traumatic brain injury (TBI) increases the risk of developing neuropsychiatric illness and cognitive impairment.. Previous work indicates that inflammatory pathways associated with interleukin (IL)-1 and interferon signaling persist in the brain chronically after TBI.. Several cell types in the brain express the interleukin-1 receptor-1 (IL1R1) including endothelia, astrocytes, and neurons.

    Staining:

    Article Title: Erythropoiesis–inosine metabolic axis failure underlying retinal neurodegeneration in glaucoma: novel diagnoses and therapies
    Article Snippet: .. Following staining, the cells were washed with flow buffer and resuspended in 100 μl of flow buffer, and analyzed within 1 h using spectral flow cytometry (Cytek Northern Lights). .. Mix I included Pacific Blue-conjugated lineage markers (BioLegend 133310), c-KIT (BD 553355), Sca-1 (BioLegend 108134), CD16/32 (BD 570676), CD34 (BD 560230), CD135 (BD 562537) and CD127 (BD 560733).

    Article Title: Erythropoiesis-inosine metabolic axis failure underlying retinal neurodegeneration in glaucoma: novel diagnoses and therapies.
    Article Snippet: .. Following staining, the cells were washed with flow buffer and resuspended in 100 μl of flow buffer, and analyzed within 1 h using spectral flow cytometry (Cytek Northern Lights). .. Mix I included Pacific Blue-conjugated lineage markers (BioLegend 133310), c-KIT (BD 553355), Sca-1 (BioLegend 108134), CD16/ 32 (BD 570676), CD34 (BD 560230), CD135 (BD 562537) and CD127 (BD 560733).

    Northern Blot:

    Article Title: Erythropoiesis–inosine metabolic axis failure underlying retinal neurodegeneration in glaucoma: novel diagnoses and therapies
    Article Snippet: .. Following staining, the cells were washed with flow buffer and resuspended in 100 μl of flow buffer, and analyzed within 1 h using spectral flow cytometry (Cytek Northern Lights). .. Mix I included Pacific Blue-conjugated lineage markers (BioLegend 133310), c-KIT (BD 553355), Sca-1 (BioLegend 108134), CD16/32 (BD 570676), CD34 (BD 560230), CD135 (BD 562537) and CD127 (BD 560733).

    Article Title: Erythropoiesis-inosine metabolic axis failure underlying retinal neurodegeneration in glaucoma: novel diagnoses and therapies.
    Article Snippet: .. Following staining, the cells were washed with flow buffer and resuspended in 100 μl of flow buffer, and analyzed within 1 h using spectral flow cytometry (Cytek Northern Lights). .. Mix I included Pacific Blue-conjugated lineage markers (BioLegend 133310), c-KIT (BD 553355), Sca-1 (BioLegend 108134), CD16/ 32 (BD 570676), CD34 (BD 560230), CD135 (BD 562537) and CD127 (BD 560733).

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro.
    Article Snippet: .. Fluorescence was then acquired using spectral flow cytometry (Cytek Northern Lights, Cytek; Fremont, CA) and analyzed using FlowJo (version 10.10). ..

    Fluorescence:

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro.
    Article Snippet: .. Fluorescence was then acquired using spectral flow cytometry (Cytek Northern Lights, Cytek; Fremont, CA) and analyzed using FlowJo (version 10.10). ..



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    (A) Experimental design. (B-D) Relative fold-change hippocampal gene expression of Cxcl1, Ccl2, Il1b . (E) Representative flow <t>cytometry</t> plots for Ly6C + monocytes, Ly6G + neutrophils and CD11c + dendritic cells. (F) Back-gating strategy to identify IL-1β-producing innate immune cells. (G-K) Bar graphs illustrate the absolute numbers of Ly6G + neutrophils (G), Ly6C + monocytes (H), CD11c + dendritic cells (I), IL-1β-producing neutrophils (J), IL-1β-producing monocytes (K). Data = Mean ± SEM (n=3-4 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 3hpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 6hpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 12hpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons test. Abbreviations: Hp, hippocampus.
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    Assessing spectral resolvability of metabolic probes for multiparametric <t>cytometry</t> (A) Experimental workflow for evaluating probe co-detection. (B and D) Representative density plots demonstrating spectral overlap for pairwise probe combinations within the (B) FITC and (D) PE channel. (C and E) Complementary validation matrices showing computational spectral similarity scores from FluoroFinder (lower left quadrant) versus empirical resolvability determined by unmixing (upper right quadrant). Gray indicates non-resolvable pairs; colored tiles indicate resolvable pairs (C, green; E, red).
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    Image Search Results


    (A) Experimental design. (B-D) Relative fold-change hippocampal gene expression of Cxcl1, Ccl2, Il1b . (E) Representative flow cytometry plots for Ly6C + monocytes, Ly6G + neutrophils and CD11c + dendritic cells. (F) Back-gating strategy to identify IL-1β-producing innate immune cells. (G-K) Bar graphs illustrate the absolute numbers of Ly6G + neutrophils (G), Ly6C + monocytes (H), CD11c + dendritic cells (I), IL-1β-producing neutrophils (J), IL-1β-producing monocytes (K). Data = Mean ± SEM (n=3-4 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 3hpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 6hpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 12hpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons test. Abbreviations: Hp, hippocampus.

    Journal: bioRxiv

    Article Title: Impact of innate immune activation on T cell dynamics and functional recovery following traumatic brain injury

    doi: 10.64898/2026.03.23.713833

    Figure Lengend Snippet: (A) Experimental design. (B-D) Relative fold-change hippocampal gene expression of Cxcl1, Ccl2, Il1b . (E) Representative flow cytometry plots for Ly6C + monocytes, Ly6G + neutrophils and CD11c + dendritic cells. (F) Back-gating strategy to identify IL-1β-producing innate immune cells. (G-K) Bar graphs illustrate the absolute numbers of Ly6G + neutrophils (G), Ly6C + monocytes (H), CD11c + dendritic cells (I), IL-1β-producing neutrophils (J), IL-1β-producing monocytes (K). Data = Mean ± SEM (n=3-4 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 3hpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 6hpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 12hpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons test. Abbreviations: Hp, hippocampus.

    Article Snippet: All flow cytometric data were acquired using Cytek Aurora Spectral flow cytometry with SpectroFlo software.

    Techniques: Gene Expression, Flow Cytometry

    (A) Experimental design. (B-D) Relative fold-change expression levels of Cxcl10, Icam1, Itgb2 . (E) Flow cytometry plots of CD4 + , CD8 + , γδ + , and NK + T cells. (F) Representative flow cytometry plots for IL-17 and IFN-γ production by different T cell subsets. (G-O) Bar graphs illustrate the absolute numbers of CD4+ T cells (G), CD8+ T cells (H), NK + T cells (I), γδ + T cells (J), IFN-γ-producing CD4 + T cells (K), IFN-γ-producing CD8 + T cells (L), IFN-γ-producing NK + T cells (M), IL-17-producing γδ + T cells (N), and IFN-γ-producing γδ + T cells (O). Data = Mean ± SEM (n=6 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 1dpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 3dpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 10dpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons. Abbreviations: Hp, hippocampus.

    Journal: bioRxiv

    Article Title: Impact of innate immune activation on T cell dynamics and functional recovery following traumatic brain injury

    doi: 10.64898/2026.03.23.713833

    Figure Lengend Snippet: (A) Experimental design. (B-D) Relative fold-change expression levels of Cxcl10, Icam1, Itgb2 . (E) Flow cytometry plots of CD4 + , CD8 + , γδ + , and NK + T cells. (F) Representative flow cytometry plots for IL-17 and IFN-γ production by different T cell subsets. (G-O) Bar graphs illustrate the absolute numbers of CD4+ T cells (G), CD8+ T cells (H), NK + T cells (I), γδ + T cells (J), IFN-γ-producing CD4 + T cells (K), IFN-γ-producing CD8 + T cells (L), IFN-γ-producing NK + T cells (M), IL-17-producing γδ + T cells (N), and IFN-γ-producing γδ + T cells (O). Data = Mean ± SEM (n=6 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 1dpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 3dpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 10dpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons. Abbreviations: Hp, hippocampus.

    Article Snippet: All flow cytometric data were acquired using Cytek Aurora Spectral flow cytometry with SpectroFlo software.

    Techniques: Expressing, Flow Cytometry

    Assessing spectral resolvability of metabolic probes for multiparametric cytometry (A) Experimental workflow for evaluating probe co-detection. (B and D) Representative density plots demonstrating spectral overlap for pairwise probe combinations within the (B) FITC and (D) PE channel. (C and E) Complementary validation matrices showing computational spectral similarity scores from FluoroFinder (lower left quadrant) versus empirical resolvability determined by unmixing (upper right quadrant). Gray indicates non-resolvable pairs; colored tiles indicate resolvable pairs (C, green; E, red).

    Journal: Cell Reports Methods

    Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

    doi: 10.1016/j.crmeth.2026.101330

    Figure Lengend Snippet: Assessing spectral resolvability of metabolic probes for multiparametric cytometry (A) Experimental workflow for evaluating probe co-detection. (B and D) Representative density plots demonstrating spectral overlap for pairwise probe combinations within the (B) FITC and (D) PE channel. (C and E) Complementary validation matrices showing computational spectral similarity scores from FluoroFinder (lower left quadrant) versus empirical resolvability determined by unmixing (upper right quadrant). Gray indicates non-resolvable pairs; colored tiles indicate resolvable pairs (C, green; E, red).

    Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

    Techniques: Cytometry, Biomarker Discovery

    Validation framework for spectral co-resolvability of metabolic probes and fluorophore conjugates (A) Experimental workflow for co-detection assessment. (B, D, and F) Representative flow cytometry density plots demonstrating pairwise mixing between metabolic probes and fluorophores in the (B) FITC/AF488, (D) PE, and (F) APC/AF647 channel. (C, E, and G) Validation matrices per channel: upper, computational spectral similarity by FluoroFinder; lower, empirically determined resolvability. Gray tiles indicate non-resolvable pairs; colored tiles confirm resolvable pairs. (C) FITC/AF488, green; (E) PE, red; and (G) APC/AF647, blue.

    Journal: Cell Reports Methods

    Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

    doi: 10.1016/j.crmeth.2026.101330

    Figure Lengend Snippet: Validation framework for spectral co-resolvability of metabolic probes and fluorophore conjugates (A) Experimental workflow for co-detection assessment. (B, D, and F) Representative flow cytometry density plots demonstrating pairwise mixing between metabolic probes and fluorophores in the (B) FITC/AF488, (D) PE, and (F) APC/AF647 channel. (C, E, and G) Validation matrices per channel: upper, computational spectral similarity by FluoroFinder; lower, empirically determined resolvability. Gray tiles indicate non-resolvable pairs; colored tiles confirm resolvable pairs. (C) FITC/AF488, green; (E) PE, red; and (G) APC/AF647, blue.

    Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

    Techniques: Biomarker Discovery, Flow Cytometry

    Validation of three metabolic probes for simultaneous assessment of mitochondrial activity and oxidative stress (A) Representative flow cytometry density plots demonstrating spectral resolution of co-stained probes. (B) Validation matrix: lower left quadrant, computational spectral similarity by FluoroFinder; upper right quadrant, empirical resolvability determination (red: resolvable pairs). (C) Mean fluorescence intensity (MFI; mean ± SD) of individual probes in control versus rotenone/antimycin A (Rot/AA)-treated groups ( n = 6). (D) Correlation analysis of MFI between single-stain and multiplexed conditions across probes (Spearman’s r). (E) MFI of probes (mean ± SD) in control versus EZH2-knockdown (EZH2-sh) groups under single and multiplexed staining. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001.

    Journal: Cell Reports Methods

    Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

    doi: 10.1016/j.crmeth.2026.101330

    Figure Lengend Snippet: Validation of three metabolic probes for simultaneous assessment of mitochondrial activity and oxidative stress (A) Representative flow cytometry density plots demonstrating spectral resolution of co-stained probes. (B) Validation matrix: lower left quadrant, computational spectral similarity by FluoroFinder; upper right quadrant, empirical resolvability determination (red: resolvable pairs). (C) Mean fluorescence intensity (MFI; mean ± SD) of individual probes in control versus rotenone/antimycin A (Rot/AA)-treated groups ( n = 6). (D) Correlation analysis of MFI between single-stain and multiplexed conditions across probes (Spearman’s r). (E) MFI of probes (mean ± SD) in control versus EZH2-knockdown (EZH2-sh) groups under single and multiplexed staining. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001.

    Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

    Techniques: Biomarker Discovery, Activity Assay, Flow Cytometry, Staining, Fluorescence, Control, Knockdown